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Arjan Diepstra
dr.

As a hematopathologist, I work on diagnostics of all types of hematological malignancies using a comprehensive panel of different techniques. Moreover, my main research interest involves Hodgkin lymphoma, with a strong focus on interactions between tumor cells and the microenvironment. In addition, I also have a long standing interest in genetic susceptibility. My areas of expertise are: immunology, tumor cell biology, genetic association studies and molecular diagnostics in pathology. I actively participate in international (EORTC) and national (HOVON) clinical trials.

CD58 mutations are common in Hodgkin lymphoma cell lines and loss of CD58 expression in tumor cells occurs in Hodgkin lymphoma patients who relapse
Published in: Genes & Immunity
Access to document 10.1038/gene.2016.30
CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells. In diffuse large B-cell lymphoma, mutations of the CD58 gene are reported to contribute to immune evasion of the tumor cells. We previously showed CD58 mutations in three Hodgkin lymphoma (HL) cell lines by whole-exome sequencing. In this study, we confirmed the mutations by Sanger sequencing at the DNA and RNA level and showed low levels or total loss of CD58 mRNA expression in two of the three...
F. R. Abdul Razak, A. Diepstra, L. Visser, A. Berg, van den
Long Noncoding RNA Expression Profiling in Normal B-Cell Subsets and Hodgkin Lymphoma Reveals Hodgkin and Reed-Sternberg Cell Specific Long Noncoding RNAs
Published in: The American Journal of Pathology
Access to document 10.1016/j.ajpath.2016.05.011
Hodgkin lymphoma (HL) is a malignancy of germinal center (GC) B-cell origin. To explore the role of long noncoding RNAs (lncRNAs) in HL, we studied LncRNA expression patterns in normal B-cell subsets, HL cell lines, and tissues. Naive and memory B cells showed a highly similar lncRNA expression pattern, distinct from GC-B cells. Significant differential expression between I-IL and normal GC-B cells was observed for 475 lncRNA loci. For two validated lncRNAs, an enhanced expression was observed in HL, diffuse large 6-cell lymphoma, and lymphoblastoid cell lines. For...
Mina Masoumeh Tayari, Melanie Winkle, Gertrud Kortman, Jantine Sietzema, Debora de Jong, Martijn Terpstra, Pieter Mestdagh, Frans G. M. Kroese, Lydia Visser, Arjan Diepstra, Klaas Kok, Anke van den Berg, Joost Kluiver
The microenvironment of classical Hodgkin lymphoma: heterogeneity by Epstein-Barr virus presence and location within the tumor
Published in: Blood cancer journal
Access to document 10.1038/bcj.2016.26 document
R Wu, A Sattarzadeh, Bea Rutgers, A Diepstra, Anke van den Berg, Lydia Visser
Inhibition of the miR-155 target NIAM phenocopies the growth promoting effect of miR-155 in B-cell lymphoma
Published in: Oncotarget
Access to document 10.18632/oncotarget.6165 document
Several studies have indicated an important role for miR-155 in the pathogenesis of B-cell lymphoma. Highly elevated levels of miR-155 were indeed observed in most B-cell lymphomas with the exception of Burkitt lymphoma (BL). However, the molecular mechanisms that underlie the oncogenic role of miR-155 in B-cell lymphoma are not well understood. To identify the miR-155 targets relevant for B-cell lymphoma, we performed RNA immunoprecipitation of Argonaute 2 in Hodgkin lymphoma (HL) cells upon miR-155 inhibition and in BL cells upon ectopic expression of miR-155. We identified 54...
Joost Kluiver, Izabella Slezak-Prochazka, Debora de Jong, Katarzyna Smigielska, Gertrud Kortman, Melanie Winkle, Bea Rutgers, Jasper Koerts, Lydia Visser, Arjan Diepstra, Bart-Jan Kroesen, Anke van den Berg
Proteomics Based Identification of Proteins with Deregulated Expression in B Cell Lymphomas
Published in: PLoS ONE
Access to document 10.1371/journal.pone.0146624 document
Follicular lymphoma and diffuse large B cell lymphomas comprise the main entities of adult B cell malignancies. Although multiple disease driving gene aberrations have been identified by gene expression and genomic studies, only a few studies focused at the protein level. We applied 2 dimensional gel electrophoresis to compare seven GC B cell non Hodgkin lymphoma (NHL) cell lines with a lymphoblastoid cell line (LCL). An average of 130 spots were at least two folds different in intensity between NHL cell lines and the LCL. We selected approximately...
Rui Wu, Marcel Nijland, Bea Rutgers, Rianne Veenstra, Myra Langendonk, Lotte E. van der Meeren, Philip M. Kluin, Guanwu Li, Arjan Diepstra, Jen-Fu Chiu, Anke van den Berg, Lydia Visser