OBJECTIVE: Polymyalgia rheumatica (PMR) and giant cell arteritis (GCA) are closely related chronic inflammatory diseases in which macrophages play a central role in the pathogenesis. This study compared macrophage-related immune profiles in subacromial bursal tissues affected by PMR and temporal arteries affected by GCA to identify shared therapeutic targets. METHODS: Subacromial bursa biopsies (SABBs) were obtained from patients with active PMR (n = 11). Temporal artery biopsies (TABs) were collected from 14 patients with GCA. Immunohistochemical staining was performed for macrophage markers [CD68, CD64, CD86, CD206 and folate receptor (FR)...

Introduction: Kidney transplant recipients (KTR) have excess mortality compared to the general population and show altered creatine homeostasis. Creatine, an endogenous nitrogenous compound, is essential for energy metabolism. We investigated determinants of circulating creatine and their sex-specific associations with mortality in KTR. Methods: Intra-erythrocyte and plasma creatine were quantified by nuclear magnetic resonance in samples from KTR and living kidney donors (healthy controls) from the TransplantLines Biobank and Cohort study (ClinicalTrials.gov: NCT03272841). Determinants of intra-erythrocyte and plasma creatine were assessed using linear regression; associations with mortality were evaluated...

Rationale & Objective: A multitude of symptoms may contribute to low health-related quality of life (HRQoL) in kidney transplant recipients (KTR). We aimed to identify the most occurring and distressing symptoms, to explore potential determinants of symptom burden, and to examine associations with patient-reported outcomes in KTR. Study Design: A cross-sectional retrospective patient-reported outcome measures study. Setting & Participants: Stable KTR ≥1 year after transplantation participating in the TransplantLines Biobank and Cohort Studies. Predictors: Clinical variables, including sex, age, and time after transplantation. Outcomes: Symptom occurrence/distress/burden, medication adherence,...

The response to salvage chemotherapy in relapsed/refractory (R/R) diffuse large B-cell lymphoma (DLBCL) is poor, and data on circulating tumour deoxyribonucleic acid (ctDNA) in this setting are limited. We evaluated ctDNA dynamics in 29 patients with relapsed or refractory DLBCL who received platinum-based salvage chemotherapy at the University Medical Center Groningen. In total, 124 plasma samples were analysed using low-coverage whole-genome sequencing to detect copy number alterations (CNAs) and targeted sequencing with a 115-gene panel for single- and multi-nucleotide variants (SNVs/MNVs). The complete response rate at the end...

With tumor genomic and gene-expression profiling (GEP), this study investigated the immune-molecular signatures of a unique cohort of diffuse large B-cell lymphoma of the bone (bone-DLBCL), including primary bone (PB-DLBCL, n = 52) and polyostotic-DLBCL (n = 20), in comparison to nodal DLBCLs with germinal center B-cell (GCB) phenotype (nodal-DLBCL-GCB, n = 34). PB-DLBCL and polyostotic-DLBCL shared similar genomic profiles and transcriptomic signatures, justifying their collective analysis as bone-DLBCL. Differential incidences of EZH2, HIST1H1E, and MYC aberrations (p < 0.05) confirmed the distinct oncogenic evolution between bone-DLBCL and...