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Arjan Diepstra
dr.

As a hematopathologist, I work on diagnostics of all types of hematological malignancies using a comprehensive panel of different techniques. Moreover, my main research interest involves Hodgkin lymphoma, with a strong focus on interactions between tumor cells and the microenvironment. In addition, I also have a long standing interest in genetic susceptibility. My areas of expertise are: immunology, tumor cell biology, genetic association studies and molecular diagnostics in pathology. I actively participate in international (EORTC) and national (HOVON) clinical trials.

Functional analysis to elucidate the susceptibility mechanisms of SNPs at the REL, GATA3 and TCF3 Loci in classical Hodgkin Lymphoma
Genome Wide Association Studies (GWAS) revealedsignificant associations for SNPs mapping at the REL, GATA3 andTCF3 loci with cHL susceptibility. In this study, we aim to elucidatethe susceptibility mechanism of these associations by establishingpossible expression quantitative trait loci (eQTL) effects. Methods.EQTL analysis was performed in EBV transformed lymphoblastoidcell lines (LCLs) generated from 96 controls and 70 former cHLpatients as well as in three previously published large gene expressionstudies of HRS cells and total HL tissue samples. Results. An eQTLeffect for REL was found using cHL total tissue array data...
R. Veenstra, J. Kluiver, D. de Jong, J. Koerts, Lydia Visser, A. Diepstra
Hodgkin Reed Sternberg cells in classical hodgkin lymphoma are surrounded by t regulary cells while lymphocyte predominant cells of nodular lymphocite predominant hodgkin lymphoma are surrounded by t follicular helper cell
Lydia Visser, A. Sattarzadeh, R. Wu, B. Rutgers, A. Diepstra, Anke Berg, van den
Disturbed antigen presentation in classical Hodgkin Lymphoma; implications for immune checkpoint inhibitor therapy
Immune checkpoint inhibitors are being tested in clinical trials and show great promise in the treatment of classical Hodgkin lymphoma (cHL). The proposed mechanism of action of these inhibitors consists of reactivating T lymphocytes that have become unresponsive as a consequence of inhibitory mechanisms exerted by the tumor cells. These reactivated T cells are expected to kill tumor cells after recognizing tumor cell derived antigens that are presented by Human Leukocyte Antigens (HLA) at the tumor cell surface. Consequently, lack of tumor cell surface expression of HLA may...
M. Nijland, Lydia Visser, Rianne Veenstra, K. Kushekhar, G. van Imhoff, Anke Berg, van den, A. Diepstra
The Phenotype of Macrophages in the Inflamed Vascular Wall of Giant Cell Arteritis Resembles the Phenotype of Non-Classical Monocytes
Yannick van Sleen, Qi Wang, Wayel H. Abdulahad, Arjan Diepstra, Annemieke M. H. Boots, Elisabeth Brouwer
CD58 mutations are common in Hodgkin lymphoma cell lines and loss of CD58 expression in tumor cells occurs in Hodgkin lymphoma patients who relapse
Published in: Genes & Immunity
Access to document 10.1038/gene.2016.30
CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells. In diffuse large B-cell lymphoma, mutations of the CD58 gene are reported to contribute to immune evasion of the tumor cells. We previously showed CD58 mutations in three Hodgkin lymphoma (HL) cell lines by whole-exome sequencing. In this study, we confirmed the mutations by Sanger sequencing at the DNA and RNA level and showed low levels or total loss of CD58 mRNA expression in two of the three...
F. R. Abdul Razak, A. Diepstra, L. Visser, A. Berg, van den