Joost Kluiver
dr.
I have a central role in all research projects focusing on the role of noncoding RNAs. My main aim is to understanding how small and long non-coding RNAs contribute to the pathogenesis of B-cell lymphoma. Ongoing studies include MYC-regulated miRNAs and lncRNAs, as well as miRNA-lncRNA interactions and the role of circular RNAs. We apply state-of-the-art methodology including AGO2-RIP, RNA-FISH and gain- and loss-of-function screens using shRNA and CRISPR-Cas technology.
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(11)
Comprehensive analysis of miRNA expression in T-cell subsets of rheumatoid arthritis patients reveals defined signatures of naive and memory Tregs
Published in: Genes & Immunity
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10.1038/gene.2013.69
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Disturbed expression of microRNAs (miRNAs) in regulatory T cells (Tregs) leads to development of autoimmunity in experimental mouse models. However, the miRNA expression signature characterizing Tregs of autoimmune diseases, such as rheumatoid arthritis (RA) has not been determined yet. In this study, we have used a microarray approach to comprehensively analyze miRNA expression signatures of both naive Tregs (CD4+CD45RO-CD25++) and memory Tregs (CD4+CD45RO+CD25++), as well as conventional naive (CD4+CD45RO+CD25+) and memory (CD4+CD45RO+CD25+) T cells (Tconvs) derived from peripheral blood of RA patients and matched healthy controls. Differential expression...
Katarzyna Smigielska-Czepiel, A. van den Berg, P. Jellema, R. J. van der Lei, J. Bijzet, J. Kluiver, A. M. H. Boots, Liesbeth Brouwer, B-J Kroesen
Long Non-Coding RNAs As Components Of The MYC Network In B Cell Lymphoma
Joost Kluiver, Melanie Winkle, Mina Tayari, Martijn Terpstra, Gertrud Kortman, Debora de Jong, Jantine Sietzema, Bart Kroesen, Klaas Kok, Anke van den Berg
Long Non-Coding RNAs Are Commonly Deregulated In Hodgkin Lymphoma
Masoumeh Tayari, Klaas Kok, Gertrud Kortman, Jantine Sietzema, Debora de Jang, Martijn Terpstra, Lydia Visser, Arjan Diepstra, Joost Kluiver, Anke Van den Berg
The Role of MicroRNAs in Hodgkin’s Lymphoma
Published in: MicroRNAs in Medicine
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10.1002/9781118300312.ch26
In this chapter, we summarize the current knowledge on microRNA (miRNA) expression, function and putative clinical value in Hodgkin’s lymphoma (HL). HL is the second most common lymphoma subtype, and mainly affects young adults or elderly. Malignant cells in HL are scarce, usually less than 1%, and these cells are of B-cell origin. As miRNAs are involved in almost all known biological processes, deregulation of miRNAs is thought to play an important role in the pathogenesis of HL. MiRNA expression studies have been performed on HL cell lines,...
Dual Role of miR-21 in CD4+T-Cells: Activation-Induced miR-21 Supports Survival of Memory T-Cells and Regulates CCR7 Expression in Naive T-Cells
Published in: PLoS ONE
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10.1371/journal.pone.0076217
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Immune cell-type specific miRNA expression patterns have been described but the detailed role of single miRNAs in the function of T-cells remains largely unknown. We investigated the role of miR-21 in the function of primary human CD4+ T-cells. MiR-21 is substantially expressed in T-cells with a memory phenotype, and is robustly upregulated upon alpha CD3/CD28 activation of both naive and memory T-cells. By inhibiting the endogenous miR-21 function in activated naive and memory T-cells, we showed that miR-21 regulates fundamentally different aspects of T-cell biology, depending on the...
Katarzyna Smigielska-Czepiel, Anke van den Berg, Pytrick Jellema, Izabella Slezak-Prochazka, Henny Maat, Hilda van den Bos, Roelof Jan van der Lei, Joost Kluiver, Elisabeth Brouwer, Anne Mieke H. Boots, Bart-Jan Kroesen