Joost Kluiver
dr.
I have a central role in all research projects focusing on the role of noncoding RNAs. My main aim is to understanding how small and long non-coding RNAs contribute to the pathogenesis of B-cell lymphoma. Ongoing studies include MYC-regulated miRNAs and lncRNAs, as well as miRNA-lncRNA interactions and the role of circular RNAs. We apply state-of-the-art methodology including AGO2-RIP, RNA-FISH and gain- and loss-of-function screens using shRNA and CRISPR-Cas technology.
Latest publications
(86)
Activities
(27)
Press/Media
(1)
Prizes
(1)
Datasets
(3)
Supervised work
(10)
Mutations in CD58 and MYB in Hodgkin Lymphoma
F.R. Abdul Razak, A. Diepstra, D. Jong, J. Koerts, B. Rutgers, J. Kluiver, Lydia Visser, Anke Berg, van den
Functional analysis to elucidate the susceptibility mechanisms of SNPs at the REL, GATA3 and TCF3 Loci in classical Hodgkin Lymphoma
Genome Wide Association Studies (GWAS) revealedsignificant associations for SNPs mapping at the REL, GATA3 andTCF3 loci with cHL susceptibility. In this study, we aim to elucidatethe susceptibility mechanism of these associations by establishingpossible expression quantitative trait loci (eQTL) effects. Methods.EQTL analysis was performed in EBV transformed lymphoblastoidcell lines (LCLs) generated from 96 controls and 70 former cHLpatients as well as in three previously published large gene expressionstudies of HRS cells and total HL tissue samples. Results. An eQTLeffect for REL was found using cHL total tissue array data...
Long Noncoding RNA Expression Profiling in Normal B-Cell Subsets and Hodgkin Lymphoma Reveals Hodgkin and Reed-Sternberg Cell Specific Long Noncoding RNAs
Published in: The American Journal of Pathology
Access to document
10.1016/j.ajpath.2016.05.011
Hodgkin lymphoma (HL) is a malignancy of germinal center (GC) B-cell origin. To explore the role of long noncoding RNAs (lncRNAs) in HL, we studied LncRNA expression patterns in normal B-cell subsets, HL cell lines, and tissues. Naive and memory B cells showed a highly similar lncRNA expression pattern, distinct from GC-B cells. Significant differential expression between I-IL and normal GC-B cells was observed for 475 lncRNA loci. For two validated lncRNAs, an enhanced expression was observed in HL, diffuse large 6-cell lymphoma, and lymphoblastoid cell lines. For...
Mina Masoumeh Tayari, Melanie Winkle, Gertrud Kortman, Jantine Sietzema, Debora de Jong, Martijn Terpstra, Pieter Mestdagh, Frans G. M. Kroese, Lydia Visser, Arjan Diepstra, Klaas Kok, Anke van den Berg, Joost Kluiver
Functional Studies on Primary Tubular Epithelial Cells Indicate a Tumor Suppressor Role of SETD2 in Clear Cell Renal Cell Carcinoma
Published in: Neoplasia
Access to document
10.1016/j.neo.2016.04.005
document
SET domain-containing 2 (SETD2) is responsible for the trimethylation of histone H3 lysine36 (H3K36me3) and is one of the genes most frequently mutated in clear cell renal cell carcinoma (ccRCC). It is located at 3p21, one copy of which is lost in the majority of ccRCC tumors, suggesting that SETD2 might function as a tumor suppressor gene. However, the manner in which loss of SETD2 contributes to ccRCC development has not been studied in renal primary tubular epithelial cells (PTECs). Therefore, we studied the consequences of SETD2 knockdown...
Jun Li, Joost Kluiver, Jan Osinga, Helga Westers, Maaike B. van Werkhoven, Marc A. Seelen, Rolf H. Sijmons, Anke van den Berg, Klaas Kok
Analysis of serum immune markers in seropositive and seronegative rheumatoid arthritis and in high-risk seropositive arthralgia patients
Published in: Scientific Reports
Access to document
10.1038/srep26021
document
Presence of autoantibodies precedes development of seropositive rheumatoid arthritis (SP RA) and seropositive arthralgia patients (SAP) are at risk of developing RA. The aims of the study are to identify additional serum immune markers discriminating between SP and seronegative (SN) RA, and markers identifying high-risk SAP. Sera from SAP (n = 27), SP RA (n = 22), SN RA (n = 11) and healthy controls (n = 20) were analyzed using the Human Cytokine 25-Plex Panel. Selected markers were validated in independent cohorts of SP RA (n =...
Paulina Chalan, Johan Bijzet, Anke van den Berg, Joost Kluiver, Bart-Jan Kroesen, Annemieke M. H. Boots, Elisabeth Brouwer