Anke van den Berg
prof. dr.
I work as a clinical molecular biologist in the department of Pathology. In this function I supervise and implement advanced molecular diagnostic techniques. Within my research line, I focus on the molecular pathogenesis of B-cell Hodgkin and non-Hodgkin lymphoma. The specific fields of interest are genomic aberrations, genetic susceptibility, and the role of small and long noncoding RNAs. I have several international collaborations and am PI and co-PI in various projects.
Comparison between one and two rounds of RNA amplification on gene expression profiles from laser-microdissected squamous cell lung carcinoma and normal bronchial epithelium
Mirjam C. Boelens, Gerard J. Te Meerman, Marie Geerlings, Harry Jm Groen, Dirkje S. Postma, Wim Timens, Anke Van den Berg
Cigarette smoke-induced emphysema: A role for the B cell?
Published in: American Journal of Respiratory and Critical Care Medicine
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10.1164/rccm.200504-594OC
Rationale: Little is known about what drives the inflammatory reaction in the development of chronic obstructive lung disease. B cells have been found. Objective: To study the involvement of B cells in the development of emphysema. Methods: The presence of B-cell follicles and their interaction with other cells were investigated in lungs of patients with chronic obstructive pulmonary disease and of smoking mice. B cells were isolated from lymphoid follicles by laser microdissection and analyzed for the presence of immunoglobulin rearrangements and somatic mutations. Main Results: Lymphoid follicles...
BWA van der Strate, DS Postma, CA Brandsma, BN Melgert, MA Luinge, M Geerlings, MN Hylkema, Anke van den Berg, W Timens, HAM Kerstjens
Lack of BIC and MicroRNA miR-155 expression in primary cases of Burkitt lymphoma
Published in: Genes Chromosomes & Cancer
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10.1002/gcc.20273
We previously demonstrated high expression of primary-microRNA BIC (pri-miR-155) in Hodgkin lymphoma (HL) and lack of expression in most non-Hodgkin lymphoma subtypes including some Burkitt lymphoma (BL) cases. Recently, high expres- sion of BIC was reported in BL in comparison to pediatric leukemia and normal peripheral-blood samples. In this study, we extended our series of BL cases and cell lines to examine expression of BIC using RNA in situ hybridization (ISH) and quantitative RT-PCR (qRT-PCR) and of miR-155 using Northern blotting. Both BIC RNA ISH and qRT-PCR revealed...
J. Kluiver, E. Haralambieva, Doetje de Jong, T. Blokzijl, S. Jacobs, Bart-Jan Kroesen, Sibrand Poppema, Anke van den Berg
Cytokine gene expression profile distinguishes CD4+/CD57+T cells of the nodular lymphocyte predominance type of Hodgkin’s lymphoma from their tonsillar counterparts
Published in: The Journal of Pathology
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10.1002/path.1894
Little is known about the cytokine profile of nodular lymphocyte predominance Hodgkin’s lymphoma (NLPHL) and the significance of the characteristic rosetting CD4(+)/CD57(+) T cells. We analysed the T lymphocyte populations isolated from lymph node suspensions from five patients with NLPHL, two with follicular hyperplasia and progressive transformation of germinal centres (PTGC), three with classical Hodgkin’s lymphoma (CHL) and five with hyperplasia of the tonsil. We sorted the T cells based on expression of CD3, CD4 and CD57 by flow cytometry and evaluated the cytokine mRNA profiles of the...
Cigdem Atayar, Sibrand Poppema, Lydia Visser, Anke van den Berg
High expression of calcium-binding proteins, S100A10, S100A11 and CALM2 in anaplastic large cell lymphoma
Published in: British Journal of Haematology
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10.1111/j.1365-2141.2005.05816.x
Anaplastic large cell lymphomas (ALCL) are characterised by the presence of CD30-positive large cells, which usually are of T-cell type. Based on the presence or absence of translocations involving the anaplastic lymphoma kinase (ALK) locus, ALCL cases can be divided into two groups. To gain more insight in the biology of ALCL, we applied serial analysis of gene expression (SAGE) on the Karpas299 cell line and identified 25 up- and 19 downregulated genes. Comparison of the differentially expressed genes with DNA copy number changes in Karpas299 revealed that...
R Rust, L Visser, J van der Leij, G Harms, T Blokzijl, JC Deloulme, P van der Vlies, W Kamps, K Kok, M Lim, S. Poppema, Anke van den Berg