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Joost Kluiver
dr.

I have a central role in all research projects focusing on the role of noncoding RNAs. My main aim is to understanding how small and long non-coding RNAs contribute to the pathogenesis of B-cell lymphoma. Ongoing studies include MYC-regulated miRNAs and lncRNAs, as well as miRNA-lncRNA interactions and the role of circular RNAs. We apply state-of-the-art methodology including AGO2-RIP, RNA-FISH and gain- and loss-of-function screens using shRNA and CRISPR-Cas technology.

Circulating miRNAs in patients with Barrett’s esophagus, high-grade dysplasia and esophageal adenocarcinoma
Published in: World journal of gastrointestinal oncology
Access to document 10.21037/jgo.2018.08.01 document
Background: Diagnostic screening of premalignant esophageal lesions is hampered by the absence of biomarkers indicative of metaplastic and/or malignant transformation. The aim of this exploratory study was to investigate the potential use of miRNAs as biomarkers capable of identifying patients with (pre)malignant lesions: Barrett’s esophagus (BE) metaplasia, high-grade dysplasia (HGD) or esophageal adenocarcinoma (EAC). Methods: A total of 69 patients were included in the study. Six serum samples from each of four study groups, i.e., patients with normal squamous epithelium (SE), BE, HGD and EAC, were profiled using...
Kirill Pavlov, Joost Kluiver, Coby Meijer, Wytske Boersma-van Ek, Frank A. E. Kruyt, Arend Karrenbeld, Jan H. Kleibeuker, Frans T. M. Peters, Anke van den Berg
Tuberous sclerosis complex is required for tumor maintenance in MYC-driven Burkitt’s lymphoma
Published in: The EMBO Journal
Access to document 10.15252/embj.201798589 document
The tuberous sclerosis complex (TSC) 1/2 is a negative regulator of the nutrient-sensing kinase mechanistic target of rapamycin complex (mTORC1), and its function is generally associated with tumor suppression. Nevertheless, biallelic loss of function of TSC1 or TSC2 is rarely found in malignant tumors. Here, we show that TSC1/2 is highly expressed in Burkitt’s lymphoma cell lines and patient samples of human Burkitt’s lymphoma, a prototypical MYC-driven cancer. Mechanistically, we show that MYC induces TSC1 expression by transcriptional activation of the TSC1 promoter and repression of miR-15a. TSC1...
Götz Hartleben, Christine Müller, Andreas Krämer, Heiko Schimmel, Laura M Zidek, Carsten Dornblut, René Winkler, Sabrina Eichwald, Gertrud Kortman, Christian Kosan, Joost Kluiver, Iver Petersen, Anke van den Berg, Zhao-Qi Wang, Cornelis F Calkhoven
Age-related gene and microRNA expression changes in the airways of healthy individuals
Published in: European Respiratory Journal
Access to document 10.1183/13993003.congress-2018.PA4230
Roy R. Woldhuis, Jennie Ong, Ilse M. Boudewijn, Anke Van den Berg, Joost L. Kluiver, Klaas Kok, Martijn M. Terpstra, Victor Guryev, Maaike De Vries, Corneel J. Vermeulen, Wim Timens, Maarten Van den Berge, Corry-Anke Brandsma
Involvement of MicroRNAs in the Aging-Related Decline of CD28 Expression by Human T Cells
Published in: Frontiers in Immunology
Access to document 10.3389/fimmu.2018.01400 document
Loss of CD28 is a characteristic feature of T cell aging, but the underlying mechanisms of this loss are elusive. As differential expression of microRNAs (miRNAs) has been described between CD28+ and CD28- T cells, we hypothesized that altered miRNA expression contributes to the age-associated downregulation of CD28. To avoid the confounding effects of age-associated changes in the proportions of T cells at various differentiation stages in vivo, an experimental model system was used to study changes over time in the expression of miRNA associated with the loss...
Nato Teteloshvili, Gerjan Dekkema, Annemieke M. Boots, Peter Heeringa, Pytrick Jellema, Debora de Jong, Martijn Terpstra, Elisabeth Brouwer, Graham Pawelec, Klaas Kok, Anke van den Berg, Joost Kluiver, Bart-Jan Kroesen
Argonaute 2 RNA Immunoprecipitation Reveals Distinct miRNA Targetomes of Primary Burkitt Lymphoma Tumors and Normal B Cells
Published in: The American Journal of Pathology
Access to document 10.1016/j.ajpath.2018.01.018 document
miRNAs are small noncoding RNAs involved in the posttranscriptional regulation of gene expression. Deregulated miRNA levels have been linked to Burkitt lymphoma (BL) pathogenesis. To date, the number of known pathogenesis-related miRNA-target gene interactions is limited. Here, we determined for the first time the miRNA targetomes of primary BL tumors and normal B cells. AG02-RNA immunoprecipitation of two frozen diagnostic BL tissue samples and three CD19(+) B-cell samples isolated from routinely removed tonsils showed distinct miRNA targetomes of BL and normal B cells. In contrast to normal B...
Agnieszka Dzikiewicz-Krawczyk, Arjan Diepstra, Bea Rutgers, Gertrud Kortman, D. de Jong, Jasper Koerts, Marian Bulthuis, Tineke van der Sluis, Annika Seitz, Lydia Visser, Klaas Kok, Joost Kluiver, Anke van den Berg