Om meer inzicht te krijgen in het ontstaan van het Hodgkin lymfoom hebben we het genexpressieprofiel van Hodgkin cellen vergeleken met normale B cellen waaruit de Hodgkin cellen ontstaan. Hieruit is gebleken dat de Hodgkin cellen qua genexpressie profiel helemaal niet meer lijken op normale B cellen. Een gen dat consistent tot expressie werd gebracht door de Hodgkin cellen was het BIC gen. Het RNA afgeschreven van dit gen bleek niet te coderen voor een eiwit maar kon worden omgezet naar het microRNA miR-155. microRNAs zijn een nieuwe...

In a previous study we demonstrated high expression of the non-coding BIC gene in the vast majority of Hodgkin’s lymphomas (HLs). Evidence suggesting that BIC is a primary rmcroRNA transcript containing the mature microRNA-155 (miR-155) as part of a RNA hairpin is now accumulating. We therefore analysed HL cell lines and tissue samples to determine whether miR-155 is also expressed in HL. High levels of miR-155 could be demonstrated, indicating that BIC is processed into a microRNA in HL. Most non-HL subtypes were negative for BIC as determined...

There are several indications that classical Hodgkin lymphoma (cHL) and at least a proportion of cases of Primary Mediastinal B cell Lymphoma (PMBL) are derived from B cells at similar stages of differentiation and share common pathogenic mechanisms. The first indication was the existence of mediastinal grey zone lymphomas as identified in the 4th International Symposium on HL, with clinical, histological and immunohistochemical features intermediate between cHL and PMBL. Second, both tumor types resemble a cell that is developmentally situated in-between the germinal center reaction and a plasma...

Aim: To gain more insight into the genes involved in the aetiology and pathogenesis of anaplastic large cell lymphoma (ALCL). Methods: Serial analysis of gene expression ( SAGE) was undertaken on the CD4+ALK+ ( anaplastic lymphoma kinase positive) ALCL derived cell line Karpas299 and as comparison on CD4+ T cells. Quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry were performed on five ALCL derived cell lines and 32 tissue samples to confirm the SAGE data. Results: High expression of Mcl-1 was seen in the Karpas299 cell line,...