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Joost Kluiver
dr.

I have a central role in all research projects focusing on the role of noncoding RNAs. My main aim is to understanding how small and long non-coding RNAs contribute to the pathogenesis of B-cell lymphoma. Ongoing studies include MYC-regulated miRNAs and lncRNAs, as well as miRNA-lncRNA interactions and the role of circular RNAs. We apply state-of-the-art methodology including AGO2-RIP, RNA-FISH and gain- and loss-of-function screens using shRNA and CRISPR-Cas technology.

Supplementary Material for: MicroRNA High Throughput Loss-of-Function Screening Reveals an Oncogenic Role for miR-21-5p in Hodgkin Lymphoma
Access to document 10.6084/m9.figshare.7047695.v1
Background/Aims: Classical Hodgkin lymphoma (cHL) is among the most frequent lymphoma subtypes. The tumor cells originate from crippled germinal center (GC)-B cells that escaped from apoptosis. MicroRNAs (miRNAs) play important roles in B-cell maturation and aberrant expression of miRNAs contributes to the pathogenesis of cHL. Our aim was to identify oncogenic miRNAs relevant for growth of cHL using a high-throughput screening approach. Methods: A lentiviral pool of 63 miRNA inhibition constructs was used to identify miRNAs essential to cell growth in three cHL cell lines in duplicate. As...
Fubiao Niu (Contributor)Ye Yuan (Second Affiliated Hospital of Harbin Medical University) (Contributor)Maria Sarkis Azkanaz (Contributor)Arjan Diepstra (Contributor)Leo Bystrykh (Contributor)Lydia Visser (Contributor)Agnieszka Dzikiewicz-Krawczyk (Polish Academy of Sciences) (Contributor)Jan Osinga (Contributor)Bea Rutgers (Contributor)Klaas Kok (Contributor)Jasper Koerts (Contributor)Martijn Terpstra (Contributor)Joost Kluiver (Contributor)Anke van den Berg (Contributor)Ilja Nolte (Contributor)Debora de Jong (Contributor)
Supplementary Material for: MicroRNA High Throughput Loss-of-Function Screening Reveals an Oncogenic Role for miR-21-5p in Hodgkin Lymphoma
Access to document 10.6084/m9.figshare.7047695
Background/Aims: Classical Hodgkin lymphoma (cHL) is among the most frequent lymphoma subtypes. The tumor cells originate from crippled germinal center (GC)-B cells that escaped from apoptosis. MicroRNAs (miRNAs) play important roles in B-cell maturation and aberrant expression of miRNAs contributes to the pathogenesis of cHL. Our aim was to identify oncogenic miRNAs relevant for growth of cHL using a high-throughput screening approach. Methods: A lentiviral pool of 63 miRNA inhibition constructs was used to identify miRNAs essential to cell growth in three cHL cell lines in duplicate. As...
Ye Yuan (Creator)Fubiao Niu (Creator)Ilja Nolte (Creator)Jasper Koerts (Creator)Bea Rutgers (Creator)Jan Osinga (Creator)Maria Sarkis Azkanaz (Creator)Martijn Terpstra (Creator)Leo Bystrykh (Creator)Arjan Diepstra (Creator)Lydia Visser (Creator)Agnieszka Dzikiewicz-Krawczyk (Polish Academy of Sciences) (Creator)Klaas Kok (Creator)Joost Kluiver (Creator)Anke van den Berg (Creator)
Inhibition of the miR-155 target NIAM phenocopies the growth promoting effect of miR-155 in B-cell lymphoma [L1236]
Several studies have indicated an important role for miR-155 in the pathogenesis of B-cell lymphoma. Highly elevated levels of miR-155 were indeed observed in most B-cell lymphomas with the exception of Burkitt lymphoma (BL). However, the molecular mechanisms that underlie the oncogenic role of miR-155 in B-cell lymphoma are not well understood. To identify the miR-155 targets relevant for B-cell lymphoma, we performed RNA immunoprecipitation of Argonaute 2 in Hodgkin lymphoma (HL) cells upon miR-155 inhibition and in BL cells upon ectopic expression of miR-155. We identified 54...
Joost Kluiver (Creator)Izabella Slezak (Creator)Anke van den Berg (Creator)