Anke van den Berg
prof. dr.
I work as a clinical molecular biologist in the department of Pathology. In this function I supervise and implement advanced molecular diagnostic techniques. Within my research line, I focus on the molecular pathogenesis of B-cell Hodgkin and non-Hodgkin lymphoma. The specific fields of interest are genomic aberrations, genetic susceptibility, and the role of small and long noncoding RNAs. I have several international collaborations and am PI and co-PI in various projects.
Brain death induces inflammation in the donor intestine
Published in: Transplantation
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10.1097/TP.0b013e31817ba53a
Background. Brain death donors are frequently used for transplantation. Previous studies showed that brain death (BD) negatively affects the immunological and inflammatory status of both liver and kidney. Because the intestine is increasingly used as a donor organ and no information on effects of BD on small intestine is available we performed this study. Methods. We studied the inflammatory and apoptotic changes in donor intestine after BD induction. Brain death was induced in rats by inflation of a balloon catheter. Three groups (n=6) were compared: 1-hr BD, 4-hr...
Lyan G. Koudstaal, Nils A. 't Hart, Petra J. Ottens, Anke van den Berg, Rutger J. Ploeg, Harry van Goor, Henri G. D. Leuvenink
Smoke-induced and COPD-related epithelial gene expression profiles: relationship with lung cancer
Mirjam Boelens, Anke van den Berg, Rudolf Fehrmann, Marie Geerlings, Wouter de Jong, Gerard te Meerman, Hannie Sietsma, Wim Timens, Dirkje Postma, Harry Groen
miRNA analysis in B-cell chronic lymphocytic leukaemia: proliferation centres characterized by low miR-150 and high BIC/milk-155 expression
Published in: The Journal of Pathology
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10.1002/path.2333
Several miRNAs have been reported to be associated with immunoglobulin heavy chain (IgH) mutation and ZAP-70 expression status in blood samples of B-cell chronic lymphocytic leukaemia/small lymphocytic lymphoma (B-CLL/SLL). In the bone marrow and lymphoid tissues, proliferation centres (PCs) represent an important site of activation and proliferation of the neoplastic cells, suggesting that these tissues better reflect the biology of CLL than circulating blood cells. We collected 33 lymph nodes and 37 blood CLL samples and analysed IgH mutation status and ZAP-70 expression status. Expression of 15 miRNAs...
M. Wang, L. P. Tan, M. K. Dijkstra, K. van Lom, J-L Robertus, G. Harms, T. Blokzijl, K. Kooistra, M. B. van t'Veer, S. Rosati, L. Visser, M. Jongen-Lavrencic, P. M. Kluin, Anke van den Berg
The CD4+CD26-T-cell population in classical Hodgkin’s lymphoma displays a distinctive regulatory T-cell profile
Published in: Laboratory Investigation
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10.1038/labinvest.2008.24
Little is known about the gene expression profile and significance of the rosetting CD4+CD26- T cells in classical Hodgkin’s lymphoma (cHL). To characterize these T cells, CD4+CD26- and CD4+CD26+ T-cell populations were sorted from lymph node (LN) cell suspensions from nodular sclerosis HL (NSHL) and reactive LNs. mRNA profiles of stimulated and resting cell subsets were evaluated with quantitative RT-PCR for 46 genes. We observed a higher percentage of CD4+CD26- T-cells in NSHL than in reactive LNs. The resting CD4+CD26-T cells in NSHL showed higher mRNA levels of...
Yue Ma, Lydia Visser, Tjasso Blokzijl, Geert Harms, Cigdem Atayar, Sibrand Poppema, Anke van den Berg
ATR alterations in Hodgkin’s lymphoma
Hodgkin’s lymphoma (HL) is characterized by the presence of neoplastic Hodgkin and Reed-Sternberg cells (HRSC) in a background of inflammatory cells. Free radicals and oxidative stress generated in the inflammatory lesions could cause DNA damage, thus providing a basis for lymphomagenesis. Ataxia-telangiectasia mutated (ATM) and Rad3-related (ATR) genes are responsive genes for DNA damage, therefore the potential involvement of the ATR gene in HL pathogenesis was examined in 8 HL cell lines and 7 clinical cases. ATR alterations were detected in 6 out of 8 HL lines. Most...
Angen Liu, Tetsuya Takakuwa, Shigeki Fujita, Wen-Juan Luo, Kristianti Tresnasari, Anke Van den Berg, Sibrand Poppema, Katsuyuki Aozasa