Anke van den Berg
prof. dr.
I work as a clinical molecular biologist in the department of Pathology. In this function I supervise and implement advanced molecular diagnostic techniques. Within my research line, I focus on the molecular pathogenesis of B-cell Hodgkin and non-Hodgkin lymphoma. The specific fields of interest are genomic aberrations, genetic susceptibility, and the role of small and long noncoding RNAs. I have several international collaborations and am PI and co-PI in various projects.
CX Chemokine Receptor 7 Contributes to Survival of KRAS-Mutant Non-Small Cell Lung Cancer upon Loss of Epidermal Growth Factor Receptor
Published in: Cancers
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10.3390/cancers11040455
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KRAS-driven non-small cell lung cancer (NSCLC) patients have no effective targeted treatment. In this study, we aimed to investigate targeting epidermal growth factor receptor (EGFR) as a therapeutic approach in KRAS-driven lung cancer cells. We show that ablation of EGFR significantly suppressed tumor growth in KRAS-dependent cells and induced significantly higher expression of CX chemokine receptor 7 (CXCR7) and activation of MAPK (ERK1/2). Conversely, rescue of EGFR led to CXCR7 downregulation in EGFR-/- cells. Dual EGFR and CXCR7 inhibition led to substantial reduction of MAPK (pERK) and synergistic...
Bin Liu, Shanshan Song, Rita Setroikromo, Siwei Chen, Wenteng Hu, Deng Chen, Anthonie J van der Wekken, Barbro N Melgert, Wim Timens, Anke van den Berg, Ali Saber, Hidde J Haisma
Age-related gene and miRNA expression changes in airways of healthy individuals
Published in: Scientific Reports
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10.1038/s41598-019-39873-0
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Knowledge on age-related miRNA changes in healthy individuals and their interaction with mRNAs is lacking. We studied age-related mRNA and miRNA expression changes and their interactions in normal airways. RNA and small RNA sequencing was performed on bronchial biopsies of 86 healthy individuals (age: 18-73) to determine age-related expression changes. Per age-related miRNA we determined the enrichment of age-related predicted targets and their correlation. We identified 285 age-related genes and 27 age-related miRNAs. Pathway enrichment showed that genes higher expressed with age were involved in synapse-related processes. Genes...
J. Ong, R. R. Woldhuis, I. M. Boudewijn, A. van den Berg, J. Kluiver, K. Kok, M. M. Terpstra, V. Guryev, M. de Vries, C. J. Vermeulen, W. Timens, M. van den Berge, C. A. Brandsma
Correction: miR-24-3p Is Overexpressed in Hodgkin Lymphoma and Protects Hodgkin and Reed Sternberg Cells from Apoptosis (vol 187, pg 1343, 2017)
Published in: American Journal of Pathology
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10.1016/j.ajpath.2018.12.002
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Ye Yuan, Joost Kluiver, Jasper Koerts, Debora de Jong, Bea Rutgers, F. Reeny Abdul Razak, Martijn Terpstra, Boudewijn E. Plaat, Ilja M. Nolte, Arjan Diepstra, Lydia Visser, Klaas Kok, Anke van den Berg
NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma
Published in: Lymphoma
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10.1007/978-1-4939-9151-8_12
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MicroRNAs (miRNAs) play important roles in development, differentiation, and homeostasis by regulating protein translation. In B-cell lymphoma, many miRNAs have altered expression levels, and for a limited subset of them, experimental data supports their functional relevance in lymphoma pathogenesis. This chapter describes an unbiased next-generation sequencing (NGS)-based high-throughput screening approach to identify miRNAs that are involved in the control of cell growth. First, we provide a protocol for performing high-throughput screening for miRNA inhibition and overexpression. Second, we describe the procedure for next-generation sequencing library preparation. Third, we...
Intricate crosstalk between MYC and non-coding RNAs regulates hallmarks of cancer
Published in: Molecular oncology
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10.1002/1878-0261.12409
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Myelocytomatosis viral oncogene homolog (MYC) plays an important role in the regulation of many cellular processes, and its expression is tightly regulated at the level of transcription, translation, protein stability, and activity. Despite this tight regulation, MYC is overexpressed in many cancers and contributes to multiple hallmarks of cancer. In recent years, it has become clear that noncoding RNAs add a crucial additional layer to the regulation of MYC and its downstream effects. So far, twenty-five microRNAs and eighteen long noncoding RNAs that regulate MYC have been identified....
Lotteke J. Y. M. Swier, Agnieszka Dzikiewicz-Krawczyk, Melanie Winkle, Anke van den Berg, Joost Kluiver