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Joost Kluiver
dr.

I have a central role in all research projects focusing on the role of noncoding RNAs. My main aim is to understanding how small and long non-coding RNAs contribute to the pathogenesis of B-cell lymphoma. Ongoing studies include MYC-regulated miRNAs and lncRNAs, as well as miRNA-lncRNA interactions and the role of circular RNAs. We apply state-of-the-art methodology including AGO2-RIP, RNA-FISH and gain- and loss-of-function screens using shRNA and CRISPR-Cas technology.

The role of microRNAs in normal hematopoiesis and hematopoietic malignancies
Published in: Leukemia
Access to document 10.1038/sj.leu.2404387
Over the past few years, it has become evident that microRNAs ( miRNAs) play an important regulatory role in various biological processes. Much effort has been put into the elucidation of their biogenesis, and this has led to the general concept that a number of key regulators are shared with the processing machinery of small interfering RNAs. Despite the recognition that several miRNAs play crucial roles in normal development and in diseases, little is known about their exact molecular function and the identity of their target genes. In...
J. Kluiver, B. -J. Kroesen, S. Poppema, Anke van den Berg
Gene expression analysis of dendritic/Langerhans cells and Langerhans cell histiocytosis
Published in: The Journal of Pathology
Access to document 10.1002/path.2003
Langerhans cell histiocytosis (LCH) is a neoplastic disorder that results in clonal proliferation of cells with a Langerhans cell (LQ phenotype. The pathogenesis of LCH is still poorly understood. In the present study, serial analysis of gene expression (SAGE) was applied to LCs generated from umbilical cord blood CD34+ progenitor cells to identify LC-specific genes and the expression of these genes in LCH was investigated. Besides the expression of several genes known to be highly expressed in LCs and LCH such as CD1a, LYZ, and CD207, high expression...
Renata Rust, J. Kluiver, Lydia Visser, G. Harms, T. Blokzijl, W.A. Kamps, Sibrand Poppema, Anke van den Berg
BCL6 alternative breakpoint region break and homozygous deletion of 17q24 in the nodular lymphocyte predominance type of Hodgkin’s lymphoma-derived cell line DEV
Published in: Human Pathology
Access to document 10.1016/j.humpath.2006.01.018
DEV is the only cell line derived from nodular lymphocyte predominance type of Hodgkin’s lymphoma (NLPHL); however, a comprehensive report about the genetic and immunophenotypic profile of this unique cell line is lacking. We analyzed DEV with respect to immunophenotype and genetic aberrations. The immunostaining revealed positivity for CD45, CD20, CD22, CD79a, IgA2, CD80, CD86, CD74, and BCL6. Cytogenetically, DEV has complex chromosome 3 translocations involving chromosomes 7, 14, and 22. A detailed analysis of the 3q27 breakpoint of the der(3)t(3;14)(p14;q32)t(3;22)(q27;q11.2) revealed a break in the BCL6 alternative...
Cigdem Atayar, K Kok, J. Kluiver, Annigje Bosga - Bouwer, Eva van den Berg, P. Van der Vlies, T. Blokzijl, G. Harms, I. Davelaar, B Sikkema-raddatz, J.I. Martin-Subero, R. Siebert, Sibrand Poppema, Anke van den Berg
Lack of BIC and MicroRNA miR-155 expression in primary cases of Burkitt lymphoma
Published in: Genes Chromosomes & Cancer
Access to document 10.1002/gcc.20273
We previously demonstrated high expression of primary-microRNA BIC (pri-miR-155) in Hodgkin lymphoma (HL) and lack of expression in most non-Hodgkin lymphoma subtypes including some Burkitt lymphoma (BL) cases. Recently, high expres- sion of BIC was reported in BL in comparison to pediatric leukemia and normal peripheral-blood samples. In this study, we extended our series of BL cases and cell lines to examine expression of BIC using RNA in situ hybridization (ISH) and quantitative RT-PCR (qRT-PCR) and of miR-155 using Northern blotting. Both BIC RNA ISH and qRT-PCR revealed...
J. Kluiver, E. Haralambieva, Doetje de Jong, T. Blokzijl, S. Jacobs, Bart-Jan Kroesen, Sibrand Poppema, Anke van den Berg
Gene expression in Hodgkin lymphoma. The role of miR-155 in normal and malignant B cells
Om meer inzicht te krijgen in het ontstaan van het Hodgkin lymfoom hebben we het genexpressieprofiel van Hodgkin cellen vergeleken met normale B cellen waaruit de Hodgkin cellen ontstaan. Hieruit is gebleken dat de Hodgkin cellen qua genexpressie profiel helemaal niet meer lijken op normale B cellen. Een gen dat consistent tot expressie werd gebracht door de Hodgkin cellen was het BIC gen. Het RNA afgeschreven van dit gen bleek niet te coderen voor een eiwit maar kon worden omgezet naar het microRNA miR-155. microRNAs zijn een nieuwe...
Joost Laurens Kluiver