Lydia Visser
PhD
My research in the Pathology department is mainly focused on immunological aspects of B-cell lymphoma. I study interactions of tumor cells with the microenvironment, and signaling pathways in Hodgkin lymphoma and non-Hodgkin lymphomas.
Neoplastic changes involving follicles: morphological, immunophenotypic and genetic diversity of lymphoproliferations derived from germinal center and mantle zone
S POPPEMA, J KALETA, J HUGH, Lydia Visser
Reactivity of monoclonal antibody B-ly7 with a subset of activated T cells and T-cell lymphomas
Antibody B-ly7 is reactive with hairy cell leukemia and a small subpopulation of normal lymphocytes. The B-ly7 antigen can also be induced on normal peripheral blood lymphocytes by phorbol ester stimulation. Recently it has been found that the reactivity pattern of B-ly7 is similar to that of HML-1, an antibody reactive with mucosal T lymphocytes and so called enteropathy associated T-cell lymphomas. Reactivity of B-ly7 with 6/61 peripheral T-cell lymphomas, including two intestinal and four extraintestinal cases, is described. The intestinal cases were CD8 and CD7 positive, whereas...
Lydia Visser, L Dabbagh, S Poppema
HML-1 ANTIGEN ON MUCOSA-ASSOCIATED T-CELLS, ACTIVATED CELLS, AND HAIRY LEUKEMIC-CELLS IS A NEW INTEGRIN CONTAINING THE BETA-7 SUBUNIT
The monoclonal antibodies HML-1, B-ly7 and Ber-ACT8 recognize intramucosal gut T lymphocytes, activated cells, and hairy cell leukemia. The antigen on hairy cells consists of three glycoproteins (160kD, 130kD and 105kD unreduced; 145kD and 120kD reduced). These peptides have biochemical features reminiscent of integrins but we have shown by immunoprecipitation that they are not known integrin subunits. We have used a newly produced antibody (BP6) to purify this molecule and shown by N-terminal sequence analysis that the smallest subunit is the product of integrin beta-7 cDNA. This molecule...
KJ MICKLEM, Yin Dong, A WILLIS, KA PULFORD, Lydia Visser, H DURKOP, S POPPEMA, H STEIN, DY MASON
SMALL LYMPHOCYTIC LYMPHOMAS WITH PREDOMINANT SPLENOMEGALY – A COMPARISON OF IMMUNOPHENOTYPES WITH CASES OF PREDOMINANT LYMPHADENOPATHY
In this study, we compared small lymphocytic lymphomas with predominant lymphadenopathy with those with predominant splenomegaly and found differences in morphology and immunophenotype as well as clinical features. Cases with lymphadenopathy were characterized by widespread disease, CLL type morphology with proliferation centers, and a CD5, CD11c, CD23, CD43 positive, CD45Ro negative immunophenotype. Cases with predominant splenomegaly had more localized disease, a mantle zone pattern or a diffuse growth pattern without proliferation centers, and a CD5, CD11c, CD23, CD43 negative, and sometimes CD45Ro positive immunophenotype. CD45Ro (UCHL1) positivity and...
H HOLLEMA, L VISSER, S POPPEMA
Monoclonal antibody OPD4 is reactive with CD45RO, but differs from UCHL1 by the absence of monocyte reactivity
Monoclonal antibody OPD4 was described as a novel monoclonal antibody recognizing a helper/inducer T-cell subset in paraffin-tissue sections. The authors have analyzed this reagent with respect to its molecular weight, reactivity with leukocyte common antigen (LCA) transfectants and cell and tissue distribution. OPD4 was found to be reactive with the 180 kd molecular weight isoform of the leukocyte common antigen family, similar to antibody UCHL1 and therefore can be considered as a CD45RO reagent. The only difference in distribution was found to be the absence of OPD4 reactivity...
S Poppema, R Lai, L. Visser