PURPOSE: The goal of this study was to assess the prognostic significance of a rearrangement of the major breakpoint region of the bcl-2 gene and/or expression of bcl-2 protein in diffuse large-cell lymphomas of B-cell origin. PATIENTS AND METHODS: All 83 patients diagnosed at the Cross Cancer Institute between 1987 and 1992 with malignant lymphoma (ML), diffuse large-cell ML non-cleaved-cell ML or cleaved-cell ML, or with diffuse large-cell immunoblastic ML were studied. bcl-2 rearrangement was identified by a polymerase chain reaction technique. This technique detects the approximately 60%...

The restricted forms of CD45 are differentially expressed on lymphocyte subsets in different maturation stages and with different functional activities. The best-known examples are the expression of CD45RA on naive and CD45RO on memory T cells. Multicolor flow cytometry allows for the analysis of lymphocyte subsets with respect to the expression of the various CD45R isoforms (RA, RB, RO). Here we report on the distribution of these isoforms in normal peripheral blood T cells, including the CD4, CD8, and CD56 subsets. The results indicate the presence of a...

Alkaline phosphatase solubilized from a human Hodgkin’s lymphoma cell line (L428) was compared with purified amphiphilic and hydrophilic forms of the enzyme from human liver, and with the enzyme solubilized from a cultured osteosarcoma cell line (Saos-2). Purified hydrophilic alkaline phosphatases from human placenta and intestine were also compared in some experiments. Alkaline phosphatase was released from the plasma membrane of intact lymphocytes by phosphatidylinositol phospholipase C and thus is anchored to the outside of the plasma membrane by covalently attached phosphatidylinositol. Enzyme released in this way was...

Alkaline phosphatase solubilized from a human Hodgkin’s lymphoma cell line (L428) was compared with purified amphiphilic and hydrophilic forms of the enzyme from human liver, and with the enzyme solubilized from a cultured osteosarcoma cell line (Saos-2). Purified hydrophilic alkaline phosphatases from human placenta and intestine were also compared in some experiments. Alkaline phosphatase was released from the plasma membrane of intact lymphocytes by phosphatidylinositol phospholipase C and thus is anchored to the outside of the plasma membrane by covalently attached phosphatidylinositol. Enzyme released in this way was...

The biology of Hodgkin’s disease is one of the most intriguing subjects in lymphoma research. The presence of only a small proportion of neoplastic cells and a vast majority of reactive cells reflects the presence of complex interactions between these cell types. In this paper we discuss findings indicating that Reed-Sternberg cells may be virally transformed cells that have evaded a cytotoxic immune response and induce an ineffective delayed type hypersensitivity reaction.